Detection of plant parasitic nematodes,Meloidogyne incognitaandPratylenchus coffeaeby multiplex PCR using specific primers
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چکیده
منابع مشابه
Selecting Degenerate Multiplex PCR Primers
Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method and make the use of SNP Genotyping more wi...
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The ease and advantages of allelespecific PCR (AS-PCR) for SNP genotyping, along with the difficulty of assay specificity with certain mismatched DNA primers, have been described in prior studies (1–3). More recently, the use of real-time PCR detection formats has been described for one or both alleles of AS-PCR (4–6). These detection enhancement methods do not overcome the inherent difficultie...
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The efficacy of six different sets of primers targeted against 16S rRNA and virulence genes such as 'iap', 'hly' and 'prf' was evaluated in separate PCR assays. The primer pairs targeted against 16S rRNA resulted into amplification of 1.2 kb PCR product. However, sets of primers targeted against different regions of 'iap' produced 371 and 660 bp PCR products, respectively. The primer pair targe...
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Procedures for using PCR to amplify novel members of gene families from genomic DNA frequently involve the use of degenerate primers. Of key importance is the ability to produce ample quantities of specific PCR product while minimizing or eliminating nonspecific side reactions. Primer design protocols and software are improving, but finding the optimal amplification conditions often involves a ...
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ژورنال
عنوان ژورنال: Soil Science and Plant Nutrition
سال: 2003
ISSN: 0038-0768,1747-0765
DOI: 10.1080/00380768.2003.10410010